Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus. 方法从人胚胎海马区分离神经干细胞,采用无血清培养基,进行体外扩增培养、传代。
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells. 方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞;
With the salt concentration increasing, contents of free proline of root, stem and leaf were increased. 各无性系Na~+含量随盐浓度的增加而逐渐上升,不同组织的Na~+含量比较根>茎>叶;
The concentration of intracellular free Ca~ ( 2+)(_i) of neurons differentiated from rat hippocampal stem cells 大鼠海马干细胞分化的神经元内钙离子浓度的测定
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord. 方法:利用无血清培养和细胞克隆培养技术,从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞,进行体外扩增培养、传代、贴壁分化观察。
Methods: To establish the methods of serum-free incubation and single-cell cloning technique, isolation and culture of neural of stem cells to acquire cell clones. 方法:采取无血清培养和单细胞克隆技术,采用原代贴壁及传代悬浮方法,培养获得细胞克隆。
Under drought and waterlogging, nitrogen supply increased the NR activity in flag leaf, the total nitrogen and free amino acid contents in leaf, stem and sheath, and the free amino acid and protein contents in grain. 水分逆境下增施氮肥提高旗叶硝酸还原酶活性、叶片与茎鞘总氮和游离氨基酸含量以及籽粒游离氨基酸和蛋白质含量。
Dynamics Changes of Soluble Sugar and Free Amino Acid Contents in Stem and Leaf of Different Oilseed Rape Varieties 油菜茎叶可溶性糖与游离氨基酸含量的动态变化《变化》简介
The content of free anthraquinone in perennial rhizome was the highest in July and September and the lowest in August, the content of free anthraquinone in annual root, young leaf, young stem and old stem were higher in July than ever. 在三年生根中游离蒽醌的含量在7月和9月出现两个峰值,8月降至最低;一年生根、幼叶、幼茎和老茎中游离蒽醌的含量在7月左右最高;
For various sentence stems, free degree which the tone fits the sentence stem is indifference, and frequency of sentence samples are also not the same. 各种句干对语气类型选择的自由度并不一致,形成的句样类型使用频度差异很大。
The experimental results showed that cycloheximide ( CHX) treatment could increase free proline content in sorghum seedlings. It may stem from: 1) inhibition of normal function of roots by CHX, resulting in water loss of sorghum seedlings; 环己亚胺(CHX)单独作用会增加高梁苗中游离脯氨酸的含量,原因可能有:一是CHX抑制了根的正常吸收功能,导致植株失水,游离脯氨酸增加;
The free space size of wheat different organ ( root, stem, the leaf, leaf sheath) has the remarkable difference. 小麦不同器官(根、茎、叶、叶鞘)自由空间的大小有显著的差异。
This could explain the reported increase of free space sugar in hormone-treated stem. 由此可进一步说明蔗糖在激素处理部位自由空间的增加。
Since excessive activation of microglial cells are associated with the release of oxygen free radicals, excitatory amino acids and inflammatory factors, and other studies also show that the autologous mobilization of neural stem cells help function recovery. 因过度活化的小胶质细胞与氧自由基、兴奋性氨基酸、炎症因子等的释放均有关系,且另有研究表明自体神经干细胞的动员亦有助于神经功能的恢复。
It is an instant and convenient assay, and can be applied to the measurement of free PC content in the grape skin, seed, stem and its extracts. 改良后的测定方法快速、简便、有效,可用于葡萄皮、籽、梗及其提取物中的游离态原花青素含量测定。